Serum Response Factor-dependent Regulation of the Smooth Muscle Calponin Gene
نویسندگان
چکیده
منابع مشابه
PKA-dependent phosphorylation of serum response factor inhibits smooth muscle-specific gene expression.
OBJECTIVE Our goal was to identify phosphorylation sites that regulate serum response factor (SRF) activity to gain a better understanding of the signaling mechanisms that regulate SRF's involvement in smooth muscle cell (SMC)-specific and early response gene expression. METHODS AND RESULTS By screening phosphorylation-deficient and mimetic mutations in SRF(-/-) embryonic stem cells, we ident...
متن کاملSmooth muscle calponin: an unconventional CArG-dependent gene that antagonizes neointimal formation.
OBJECTIVE Smooth muscle calponin (CNN1) contains multiple conserved intronic CArG elements that bind serum response factor and display enhancer activity in vitro. The objectives here were to evaluate these CArG elements for activity in transgenic mice and determine the effect of human CNN1 on injury-induced vascular remodeling. METHODS AND RESULTS Mice carrying a lacZ reporter under control o...
متن کاملIntegrative Physiology/Experimental Medicine Smooth Muscle Calponin An Unconventional CArG-Dependent Gene That Antagonizes Neointimal Formation
Objective—Smooth muscle calponin (CNN1) contains multiple conserved intronic CArG elements that bind serum response factor and display enhancer activity in vitro. The objectives here were to evaluate these CArG elements for activity in transgenic mice and determine the effect of human CNN1 on injury-induced vascular remodeling. Methods and Results—Mice carrying a lacZ reporter under control of ...
متن کاملLeiomodin 1, a new serum response factor-dependent target gene expressed preferentially in differentiated smooth muscle cells.
Smooth muscle cell (SMC) differentiation is defined largely by a number of cell-restricted genes governed directly by the serum response factor (SRF)/myocardin (MYOCD) transcriptional switch. Here, we describe a new SRF/MYOCD-dependent, SMC-restricted gene known as Leiomodin 1 (Lmod1). Conventional and quantitative RT-PCRs indicate that Lmod1 mRNA expression is enriched in SMC-containing tissue...
متن کاملExpression of Smooth Muscle Calponin in Synovial Sarcoma
Purpose. Histogenesis of synovial sarcoma remains controversial and reliable molecular markers for diagnosis are necessary. Expression of basic calponin, a smooth muscle differentiation-specific actin-binding protein, was studied in synovial sarcoma.Subjects and Methods. The basic calponin gene and the gene product were analyzed by reverse transcription PCR analysis (RT-PCR) and immunohistochem...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 2000
ISSN: 0021-9258
DOI: 10.1074/jbc.275.13.9814